Host species specificity of polyomavirus DNA replication is not altered by simian virus 40 72-base-pair repeats.

Replication but not transcription of simian virus 40 DNA is dependent on nuclear domain 10.

DNA viruses from several families including herpes simplex virus type 1, adenovirus type 5, and simian virus 40 (SV40), start their transcription and replication adjacent to a specific nuclear domain, ND10. We asked whether a specific viral DNA sequence determines the location of these synthetic activities at such restricted nuclear sites. Partial and overlapping SV40 sequences were introduced ...

Species specificity of Simian Virus 40 DNA replication in vitro requires multiple functions of human DNA polymerase α Richard

Species specificity of simian virus 40 DNA replication in vitro requires multiple functions of human DNA polymerase alpha.

Human cell extracts support the replication of SV40 DNA, whereas mouse cell extracts do not. Species specificity is determined at the level of initiation of DNA replication, and it was previously found that this requires the large subunit, p180, of DNA polymerase alpha-primase to be of human origin. Furthermore, a functional interaction between SV40 large T antigen (TAg) and p180 is essential f...

A 69-base-pair monkey DNA sequence enhances simian virus 40 replication and transcription through multiple motifs.

We have determined that a 69-base-pair (bp) monkey DNA sequence, previously found to enhance simian virus 40 replication, has transcriptional enhancer activity as well. Consensus recognition sites for the transcription factor AP-1, present at each end of this sequence, are partially responsible for its replication- and transcription-enhancing activities. Other motifs within the 69-bp monkey seq...

Relationship of replication and transcription of Simian Virus 40 DNA.

RNA produced by the Simian Virus 40 (SV40) mutant tsA30 during lytic infection of kidney cells of African green monkeys was examined by RNA-DNA competition-hybridization. This mutant is temperature-sensitive in a function (gene A) that regulates synthesis of viral DNA. No detectable difference between mutant RNA synthesized at the permissive temperature (33 degrees ) and wild-type viral RNA was...